Anabolic steroids differ in their characteristics, which means there are steroids that are only suitable for specific uses. For instance, Trenbolone is not recommended for bulking but it is an outstanding fat burner. This makes it perfect for a lean mass cycle or a cutting cycle. Always take the side effects of a particular compound into consideration whenever you plan a stack. Do not combine anabolic steroids that show similar side effects. For example, never combine Anapolon and Dianabol because they are already quite toxic and if you combine them, the toxicity will increase exponentially and cause serious damage to your body.
Detailed structural information on metabolites serving as target analytes in clinical, forensic, and sports drug testing programmes is of paramount importance to ensure unequivocal test results. In the present study, the utility of collision cross section (CCS) analysis by travelling wave ion mobility measurements to support drug metabolite characterization efforts was tested concerning recently identified glucuronic acid conjugates of the anabolic-androgenic steroid stanozolol. Employing travelling-wave ion mobility spectrometry/quadrupole-time-of-flight mass spectrometry, drift times of five synthetically derived and fully characterized steroid glucuronides were measured and subsequently correlated to respective CCSs as obtained in silico to form an analyte-tailored calibration curve. The CCSs were calculated by equilibrium structure minimization (density functional theory) using the programmes ORCA with the data set B3LYP/6-31G and MOBCAL utilizing the trajectory method (TM) with nitrogen as drift gas. Under identical experimental conditions, synthesized and/or urinary stanozolol-N and O-glucuronides were analyzed to provide complementary information on the location of glucuronidation. Finally, the obtained data were compared to CCS results generated by the system's internal algorithm based on a calibration employing a polyalanine analyte mixture. The CCSs ΩN2 calculated for the five steroid glucuronide calibrants were found between 180 and 208 Å(2) , thus largely covering the observed and computed CCSs for stanozolol-N1'-, stanozolol-N2'-, and stanozolol-O-glucuronide found at values between and Å(2) . The obtained data corroborated the earlier suggested N- and O-glucuronidation of stanozolol, and demonstrate the exploit of ion mobility and CCS computation in structure characterization of phase-II metabolic products; however, despite reproducibly measurable differences in ion mobility of stanozolol-N1'-, N2'-, and O-glucuronides, the discriminatory power of the chosen CCS computation algorithm was found to be not appropriate to allow for accurate assignments of the two N-conjugated structures. Using polyalanine-based calibrations, significantly different absolute values were obtained for all CCSs, but due to a constant offset of approximately 45 Å(2) an excellent correlation (R(2) = ) between both approaches was observed. This suggests a substantially accelerated protocol when patterns of computed and polyalanine-based experimental data can be used for structure elucidations instead of creating individual analyte-specific calibration curves.
Anabolic steroids are synthetic versions of hormones that human body produces naturally. Their main role is to assure increased physical performance in all sports and athletic pursuits. Steroidal compounds enhance stamina, strength, weight and size of muscles and may improve the energy levels during physical training. Oral and injectable steroids posses the ability to increase athletic performance, have a positive effect on red blood cells production and bones density. The steroids are used in accordance with requirement and needs of any athlete individually. There are special compounds which are not suitable for women and a series of anti-estrogen products which main role is to counter the eventual side effects of steroids use and to restore natural testosterone levels of the human body. These compounds nowadays became an important part of muscle building process among professional athletes and bodybuilders, as well as regular people, males and females that have the goal to become more attractive and exhibit good looks. Follow us on Twitter | Steroids Store - Pinterest .